The GENOVESA fastGEN software module, available online, can to be used to analyse sequencing data. It is a cloud all-in-one solution for the analysis of raw data (FASTQ format files) with technical and application support.
Compatible with MiSeq and NovaSeq sequencers, for other platforms use conversion kit.
Reconstitution
No
Applications
Isolated DNA
Sample Requirements
5 µl/well
Shipping
At -20°C temperature. Upon receipt, store the product at the temperature -20°C.
Storage/Expiration
Store the complete kit at -20°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Note
BioVendor Group fastGEN: A breakthrough system for one-step NGS library
Summary
Features
It is intended for research use only
The total assay time is less than <30 min hands on time, < 14h overal
It provides an examination of the mutation status of samples (DNA isolated ) by ultra-deep sequencing
Assay format is 96 tubes (4 tubes for one DNA sample) with Master Mixes
Components of the kit are provided ready to use
The simplicity of the fastGEN method is into mix one particular Master Mix with a DNA sample and insert it into a thermocycler.
Research topic
Others
Summary
Cystic fibrosis (CF) is a multisystem disease with autosomal recessive inheritence, affecting the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CF mainly affects the lungs and pancreas, but also the upper airways, liver, intestine, and reproductive organs.
Currently, more than 2000 germline sequence variants of the CFTR gene have already been identified, though not all result in CF.
