Type
Sandwich ELISA
Applications
Plasma
Sample Requirements
50µL
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store all components of this kit at 2-8°C. This kit is stable under these conditions for 12 months from the date of manufacturing.
Calibration Range
13.6-870 fmol/mL
Limit of Detection
1.36 fmol/mL
Features
Research Use Only.
This kit measures acylated ghrelin in plasma.
Assay format is 96 wells.
Components of the kit are provided ready-to-use, lyophilized , or concentrated.
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation
Summary
Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a peptide consisting of 28-amino acid by Dr. Kenji kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormone activity. This Desacyl-Ghrelin ELISA kit measures only the Desacyl-Ghrelin based on the principle of 2 Site Sandwich enzyme-linked immunosorbent assay (ELISA). It can detect not only human Desacyl-Ghrelin but also rat/mouse Desacyl-Ghrelin (1-28). This kit is manufactured using the high specific antibody pairs generated by Dr. Kangawa and by following his protocol. (patent pending:PCT WO 01/07475 A1)
Instructions for Use (RUO)
Instructions for Use (RUO)
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