Cystatin C Mouse ELISA

Cat. No.	Size	Price
RD291009200R	96 wells (1 kit)	$543

Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.156–10 ng/ml

Limit of Detection

0.04 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 7.1%

Inter-assay (Run-to-Run)

n = 5; CV = 8.2%

Spiking Recovery

88,70%

Dilution Linearity

104,80%

Crossreactivity

bovine Non-detectable
cat Non-detectable
dog Non-detectable
goat Non-detectable
horse Non-detectable
hamster Yes (recommended dilution 1:100)
human Non-detectable
monkey Non-detectable
pig Non-detectable
rabbit Non-detectable
sheep Non-detectable
chicken Not tested
mouse Yes
rat Yes (recommended dilution 1:200)

Summary

Features

It is intended for research use only.
The total assay time is less than 3.5 hours.
The kit measures total Cystatin C in mouse serum.
Assay format is 96 wells.
Quality Controls are animal serum based. No human sera are used.
Standard is recombinant protein based.
Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Neural tissue markers, Renal disease, Animal studies

Summary

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.

Product References (9)

References

Watson AMD, Gould EAM, Moody SC, Sivakumaran P, Sourris KC, Chow BSM, Koïtka-Weber A, Allen TJ, Jandeleit-Dahm KAM, Cooper ME, Calkin AC. Disparate Effects of Diabetes and Hyperlipidemia on Experimental Kidney Disease. Front Physiol. 2020 Jun 3;11:518. doi: 10.3389/fphys.2020.00518. eCollection 2020. PubMed PMID: 32581831. PubMed CentralPMCID: PMC7283908. See more on PubMed
Ward MS, Flemming NB, Gallo LA, Fotheringham AK, McCarthy DA, Zhuang A, Tang PH, Borg DJ, Shaw H, Harvie B, Briskey DR, Roberts LA, Plan MR, Murphy MP, Hodson MP, Forbes JM. Targeted mitochondrial therapy using MitoQ shows equivalent renoprotection to angiotensin converting enzyme inhibition but no combined synergy in diabetes. Sci Rep. 2017 Nov 9;7(1):15190. doi: 10.1038/s41598-017-15589-x. PubMed PMID: 29123192. PubMed CentralPMCID: PMC5680236. See more on PubMed
Watson AMD, Gould EAM, Penfold SA, Lambert GW, Pratama PR, Dai A, Gray SP, Head GA, Jandeleit-Dahm KA. Diabetes and Hypertension Differentially Affect Renal Catecholamines and Renal Reactive Oxygen Species. Front Physiol. 2019 Apr 16;10:309. doi: 10.3389/fphys.2019.00309. eCollection 2019. PubMed PMID: 31040788. PubMed CentralPMCID: PMC6477025. See more on PubMed
Yoshida T, Yamashita M, Horimai C, Hayashi M. Smooth Muscle-Selective Nuclear Factor-κB Inhibition Reduces Phosphate-Induced Arterial Medial Calcification in Mice With Chronic Kidney Disease. J Am Heart Assoc. 2017 Nov 16;6(11):e007248. doi: 10.1161/JAHA.117.007248. PubMed PMID: 29146611. PubMed CentralPMCID: PMC5721793. See more on PubMed
Gallo LA, Ward MS, Fotheringham AK, Zhuang A, Borg DJ, Flemming NB, Harvie BM, Kinneally TL, Yeh SM, McCarthy DA, Koepsell H, Vallon V, Pollock C, Panchapakesan U, Forbes JM. Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice. Sci Rep. 2016 May 26;6:26428. doi: 10.1038/srep26428. PubMed PMID: 27226136. PubMed CentralPMCID: PMC4881020. See more on PubMed
Rieger A, Kemter E, Kumar S, Popper B, Aigner B, Wolf E, Wanke R, Blutke A. Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle. PLoS One. 2016 Jul 15;11(7):e0158977. doi: 10.1371/journal.pone.0158977. eCollection 2016. PubMed PMID: 27420727. PubMed CentralPMCID: PMC4946790. See more on PubMed
Jha JC, Thallas-Bonke V, Banal C, Gray SP, Chow BS, Ramm G, Quaggin SE, Cooper ME, Schmidt HH, Jandeleit-Dahm KA. Podocyte-specific Nox4 deletion affords renoprotection in a mouse model of diabetic nephropathy. Diabetologia. 2016 Feb;59(2):379-89. doi: 10.1007/s00125-015-3796-0. Epub 2015 Oct 28. PubMed PMID: 26508318. PubMed CentralPMCID: PMC6450410. See more on PubMed
Voelkl J, Alesutan I, Leibrock CB, Quintanilla-Martinez L, Kuhn V, Feger M, Mia S, Ahmed MS, Rosenblatt KP, Kuro-O M, Lang F. Spironolactone ameliorates PIT1-dependent vascular osteoinduction in klotho-hypomorphic mice. J Clin Invest. 2013 Feb;123(2):812-22. doi: 10.1172/JCI64093. Epub 2013 Jan 9. PubMed PMID: 23298834. PubMed CentralPMCID: PMC3561808. See more on PubMed
Jang HR, Gandolfo MT, Ko GJ, Satpute SR, Racusen L, Rabb H. B cells limit repair after ischemic acute kidney injury. J Am Soc Nephrol. 2010 Apr;21(4):654-65. doi: 10.1681/ASN.2009020182. Epub 2010 Mar 4. PubMed PMID: 20203156. PubMed CentralPMCID: PMC2844308. See more on PubMed

Summary References (20)

References to Cystatin C

Bokenkamp A, Domanetzki M, Zinck R, Schumann G, Byrd D, Brodehl J. Cystatin C--a new marker of glomerular filtration rate in children independent of age and height. Pediatrics. 1998 May;101 (5):875-81
Delanaye P, Cavalier E, Krzesinski JM. Cystatin C, renal function, and cardiovascular risk. Ann Intern Med. 2008 Feb 19;148 (4):323
Deng A, Irizarry MC, Nitsch RM, Growdon JH, Rebeck GW. Elevation of cystatin C in susceptible neurons in Alzheimer's disease. Am J Pathol. 2001 Sep;159 (3):1061-8
Dharnidharka VR, Kwon C, Stevens G. Serum cystatin C is superior to serum creatinine as a marker of kidney function: a meta-analysis. Am J Kidney Dis. 2002 Aug;40 (2):221-6
Ekiel I, Abrahamson M, Fulton DB, Lindahl P, Storer AC, Levadoux W, Lafrance M, Labelle S, Pomerleau Y, Groleau D, LeSauteur L, Gehring K. NMR structural studies of human cystatin C dimers and monomers. J Mol Biol. 1997 Aug 15;271 (2):266-77
Luc G, Bard JM, Lesueur C, Arveiler D, Evans A, Amouyel P, Ferrieres J, Juhan-Vague I, Fruchart JC, Ducimetiere P. Plasma cystatin-C and development of coronary heart disease: The PRIME Study. Atherosclerosis. 2006 Apr;185 (2):375-80
Macisaac RJ, Tsalamandris C, Thomas MC, Premaratne E, Panagiotopoulos S, Smith TJ, Poon A, Jenkins MA, Ratnaike SI, Power DA, Jerums G. Estimating glomerular filtration rate in diabetes: a comparison of cystatin-C- and creatinine-based methods. Diabetologia. 2006 Jul;49 (7):1686-9
Mojiminiyi OA, Abdella N. Evaluation of cystatin C and beta-2 microglobulin as markers of renal function in patients with type 2 diabetes mellitus. J Diabetes Complications. 2003 May-Jun;17 (3):160-8
Moran A, Katz R, Smith NL, Fried LF, Sarnak MJ, Seliger SL, Psaty B, Siscovick DS, Gottdiener JS, Shlipak MG. Cystatin C concentration as a predictor of systolic and diastolic heart failure. J Card Fail. 2008 Feb;14 (1):19-26
Muntner P, Mann D, Winston J, Bansilal S, Farkouh ME. Serum cystatin C and increased coronary heart disease prevalence in US adults without chronic kidney disease. Am J Cardiol. 2008 Jul 1;102 (1):54-7
Muntner P, Winston J, Uribarri J, Mann D, Fox CS. Overweight, obesity, and elevated serum cystatin C levels in adults in the United States. Am J Med. 2008 Apr;121 (4):341-8
Mussap M, Plebani M. Biochemistry and clinical role of human cystatin C. Crit Rev Clin Lab Sci. 2004;41 (5-6):467-550
Nakashima I, Fujinoki M, Fujihara K, Kawamura T, Nishimura T, Nakamura M, Itoyama Y. Alteration of cystatin C in the cerebrospinal fluid of multiple sclerosis. Ann Neurol. 2007 Aug;62 (2):197-200; discussion 205
Ortiz F, Harmoinen A, Paavonen T, Koskinen P, Gronhagen-Riska C, Honkanen E. Is Cystatin C more sensitive than creatinine in detecting early chronic allograft nephropathy?. Clin Nephrol. 2008 Jul;70 (1):18-25
Parikh NI, Hwang SJ, Yang Q, Larson MG, Guo CY, Robins SJ, Sutherland P, Benjamin EJ, Levy D, Fox CS. Clinical correlates and heritability of cystatin C (from the Framingham Offspring Study). Am J Cardiol. 2008 Nov 1;102 (9):1194-8
Perlemoine C, Beauvieux MC, Rigalleau V, Baillet L, Barthes N, Derache P, Gin H. Interest of cystatin C in screening diabetic patients for early impairment of renal function. Metabolism. 2003 Oct;52 (10):1258-64
Premaratne E, MacIsaac RJ, Finch S, Panagiotopoulos S, Ekinci E, Jerums G. Serial measurements of cystatin C are more accurate than creatinine-based methods in detecting declining renal function in type 1 diabetes. Diabetes Care. 2008 May;31 (5):971-3
Risch L, Blumberg A, Huber A. Rapid and accurate assessment of glomerular filtration rate in patients with renal transplants using serum cystatin C. Nephrol Dial Transplant. 1999 Aug;14 (8):1991-6
Samouilidou EC, Grapsa E. Relationship of serum cystatin C with C-reactive protein and apolipoprotein A1 in patients on hemodialysis. Ren Fail. 2008;30 (7):711-5
Servais A, Giral P, Bernard M, Bruckert E, Deray G, Isnard Bagnis C. Is serum cystatin-C a reliable marker for metaboli

Type

Applications

Sample Requirements

Shipping

Storage/Expiration

Calibration Curve

Calibration Range

Limit of Detection

Intra-assay (Within-Run)

Inter-assay (Run-to-Run)

Spiking Recovery

Dilution Linearity

Crossreactivity

Features

Research topic

Summary

References

References to Cystatin C

Instructions for Use (RUO)

Safety Information (RUO)

Find documents for the lot

Cystatin C Mouse ELISA

Type

Applications

Sample Requirements

Shipping

Storage/Expiration

Calibration Curve

Calibration Range

Limit of Detection

Intra-assay (Within-Run)

Inter-assay (Run-to-Run)

Spiking Recovery

Dilution Linearity

Crossreactivity

Features

Research topic

Summary

References

References to Cystatin C

By Research Topic

By Molecule

By Technical Data

Instructions for Use (RUO)

Safety Information (RUO)

Find documents for the lot