Coagulation Factor VIII, Human Recombinant

Cat. No.	Size	Price
New RP1723180250	250 IU	$1496,25
New RP1723180500	500 IU	$2552,55
New RP1723181000	1000 IU	$3937,5

Technical Data

Type

Recombinant protein

Description

Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having 2332 amino acids. The Factor-VIII is purified by proprietary chromatographic techniques.

Source

CHO cells (Chinese Hamster Ovarian Cells)

Purity

Greater than 97.0% as determined by SDS-PAGE.

Biological Activity

The specific activity was found to be 7,058 IU/mg. It can vary slightly between different lots.

Formulation

The lyophilized Factor VIII was lyophilized from a solution containing 8mg Tween-80, 112mM NaCl, 40mg Mannitol, 10mg Trehalose, 1ng VWF and 4.2mM CaCl2.

Reconstitution

It is recommended to reconstitute lyophilized Factor-VIII with sterile 18M-cm H2O in 50 IU/ml concentration , which can then be further diluted to other aqueous solutions.

Shipping

2 ~ 8°C. Upon receipt, store the product at the temperature recommended below, all components are stable until the expiration date (see label on the vial).

Storage/Expiration

Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-VIII should be stored at 2-4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Note

BioVendor's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Summary

Summary

Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

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