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Chorionic Gonadotropin (hCG) Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:hCG
  • Species:Human
Cat. No. Size Price


RCD006R 96 wells (1 kit) $387,77
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2.5–500 IU/l

Limit of Detection

0.7 IU/l

Spiking Recovery

100,20%

Dilution Linearity

101,60%

Summary

Research topic

Reproduction

Summary

Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits, the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. The measurement of free β-HCG in the first trimester of pregnancy has been reported as a useful marker in antenatal screening for Down Syndrome and other fetal aneuploidies. Increased free β-HCG values in combination with maternal age, the measurement of PAPP-A and the ultrasonic determination of nuchal translucency (NT) in pregnancy weeks 11 to 14 may detect up to 90 % of pregnancies with Down syndrom.

Summary References (22)

References to Chorionic Gonadotropin

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  • Braunstein GD, Rasor J, Danzer H, Adler D, Wade ME. Serum human chorionic gonadotropin levels throughout normal pregnancy. Am J Obstet Gynecol. 1976 Nov 15;126 (6):678-81
  • Breimer L. First trimester biochemical screening for trisomy 21: the role of free beta-hCG and pregnancy associated plasma protein. Ann Clin Biochem. 1995 Mar;32 ( Pt 2):233
  • Brizot ML, Jauniaux E, Mckie AT, Farzaneh F, Nicolaides KH. Placental expression of alpha and beta subunits of human chorionic gonadotrophin in early pregnancies with Down's syndrome. Hum Reprod. 1995 Sep;10 (9):2506-9
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  • Chen R, Weng L, Sizto NC, Osorio B, Hsu CJ, Rodgers R, Litman DJ. Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin. Clin Chem. 1984 Sep;30 (9):1446-51
  • Cole LA, Hartle RJ, Laferla JJ, Ruddon RW. Detection of the free beta subunit of human chorionic gonadotropin (HCG) in cultures of normal and malignant trophoblast cells, pregnancy sera, and sera of patients with choriocarcinoma. Endocrinology. 1983 Sep;113 (3):1176-8
  • Densem J, Wald NJ. Down's syndrome screening: value of using free beta-hCG. Prenat Diagn. 1995 Jan;15 (1):94-5
  • Forest JC, Masse J, Rousseau F, Moutquin JM, Brideau NA, Belanger M. Screening for Down syndrome during the first and second trimesters: impact of risk estimation parameters. Clin Biochem. 1995 Aug;28 (4):443-9
  • Gaspard UJ, Reuter AM, Deville JL, Vrindts-Gevaert Y, Bagshawe KD, Franchimont P. Serum concentration of human chorionic gonadotropin and its alpha and beta subunits. 2. Trophoblastic tumours. Clin Endocrinol (Oxf). 1980 Oct;13 (4):319-29
  • Hay DL. Placental histology and the production of human choriogonadotrophin and its subunits in pregnancy. Br J Obstet Gynaecol. 1988 Dec;95 (12):1268-75
  • Loncar J, Barnabei VM, Larsen JW Jr. Advent of maternal serum markers for Down syndrome screening. Obstet Gynecol Surv. 1995 Apr;50 (4):316-20
  • Macri JN, Kasturi RV, Krantz DA, Cook EJ, Moore ND, Young JA, Romero K, Larsen JW Jr. Maternal serum Down syndrome screening: free beta-protein is a more effective marker than human chorionic gonadotropin. Am J Obstet Gynecol. 1990 Oct;163 (4 Pt 1):1248-53
  • Macri JN, Kasturi RV, Krantz DA, Cook EJ, Moore ND, Young JA, Romero K, Larsen JW Jr. Maternal serum Down syndrome screening: free beta-protein is a more effective marker than human chorionic gonadotropin. Am J Obstet Gynecol. 1990 Oct;163 (4 Pt 1):1248-53
  • Milwidsky A, Adoni A, Miodovnik M, Segal S, Palti Z. Human chorionic gonadotropin (beta-subunit) in the early diagnosis of ectopic pregnancy. Obstet Gynecol. 1978 Jun;51 (6):725-6
  • Miyachi Y, Vaitukaitis JL, Nieschlag E, Lipsett MB. Enzymatic radioiodination of gonadotropins. J Clin Endocrinol Metab. 1972 Jan;34 (1):23-8
  • Ozturk M, Berkowitz R, Goldstein D, Bellet D, Wands JR. Differential production of human chorionic gonadotropin and free subunits in gestational trophoblastic disease. Am J Obstet Gynecol. 1988 Jan;158 (1):193-8
  • Simpson ER, MacDonald PC. Endocrine physiology of the placenta. Annu Rev Physiol. 1981;43:163-88
  • Spencer K. Evaluation of an assay of the free beta-subunit of choriogonadotropin and its potential value in screening for Down's syndrome. Clin Chem. 1991 Jun;37 (6):809-14
  • Spencer K, Macri JN. Early detection of Down's syndrome using free beta human choriogonadotropin. Ann Clin Biochem. 1992 May;29 ( Pt 3):349-50
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
  • Vaitukaitis JL, Braunstein GD, Ro
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