Type
Sandwich ELISA
Applications
Serum, Urine, Plasma
Sample Requirements
10µl/well (urine), 5 µl/well (serum, plasma)
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
1 - 40 ng/ml
Limit of Detection
20 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 4.9%
Inter-assay (Run-to-Run)
n = 8,
CV = 6.3%
Spiking Recovery
93%
Dilution Linearity
98%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures canine and feline clusterin
Assay format is 96 wells
Quality Controls are serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Neural tissue markers, Oncology, Others, Renal disease, Sepsis
Summary
Clusterin, also known as apolipoprotein J, is a widely expressed heterodimeric glycoprotein, important in tumorigenesis, apoptosis and immunoregulation. Clusterin is a cellular chaperon that stabilizes stressed proteins in a folding-competent state and protein has also been implicated in programmed cell death. Another defining prominent of clusterin is its induction in many severe physiological disturbance states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer, and several neurodegenerative conditions (Alzheimer’s disease).
Recent findings demonstrate that high serum clusterin levels are connected to oxidation stress, vascular damage, sepsis and related mortality or significantly lower serum clusterin was observed in dogs with multicentric lymphoma (MLSA).
Urinary clusterin has been approved as a biomarker to monitor drug-induced proximal tubular injury in rats by the U.S. Food and Drug Administration and the European Medicines Agency. Furthermore, urinary clusterin may also help to differentiate between tubular and glomerular forms of proteinuria.
Instructions for Use (RUO)
Instructions for Use (RUO)
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