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Canine/Feline Clusterin ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Apolipoprotein J, Apo J, Aging-associated gene 4 protein, AAG4, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, SGP-2, Testosterone-repressed prostate message 2, TRPM-2
  • Species:Canine, Feline
Cat. No. Size Price


New RBL026R 96 wells (1 kit) $526,55
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Urine, Plasma

Sample Requirements

10µl/well (urine), 5 µl/well (serum, plasma)

Shipping

Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

1 - 40 ng/ml

Limit of Detection

20 pg/ml

Intra-assay (Within-Run)

n = 8 CV = 4.9%

Inter-assay (Run-to-Run)

n = 8, CV = 6.3%

Spiking Recovery

93%

Dilution Linearity

98%

Summary

Features

It is intended for research use only The total assay time is less than 3 hours The kit measures canine and feline clusterin Assay format is 96 wells Quality Controls are serum based Standard is a recombinant protein Components of the kit are provided ready to use, concentrated or dried

Research topic

Neural tissue markers, Oncology, Others, Renal disease, Sepsis

Summary

Clusterin, also known as apolipoprotein J, is a widely expressed heterodimeric glycoprotein, important in tumorigenesis, apoptosis and immunoregulation. Clusterin is a cellular chaperon that stabilizes stressed proteins in a folding-competent state and protein has also been implicated in programmed cell death. Another defining prominent of clusterin is its induction in many severe physiological disturbance states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer, and several neurodegenerative conditions (Alzheimer’s disease).
Recent findings demonstrate that high serum clusterin levels are connected to oxidation stress, vascular damage, sepsis and related mortality or significantly lower serum clusterin was observed in dogs with multicentric lymphoma (MLSA). Urinary clusterin has been approved as a biomarker to monitor drug-induced proximal tubular injury in rats by the U.S. Food and Drug Administration and the European Medicines Agency. Furthermore, urinary clusterin may also help to differentiate between tubular and glomerular forms of proteinuria.

Summary References (13)

References to Clusterin

  • Choi-Miura NH, Oda T: Relationship between multifunctional protein Clusterin and Alzheimer disease. Neurobiol. Aging 1996; 17(5): 717–722
  • Newkirk MM, Apostolakos P, Neville C and Fortin PR: Systemic lupus erythematosus, a disease associated with low levels of Clusterin/ApoJ, and anti-inflammatory protein. J. Rheumatol.1999; 3:597–603
  • Morrissey C, Lakins J, Moquin A, Hussain M, Tenniswood M: An antigen capture assay for the measurement of serum Clusterin concentrations. J. Biochen. Biophys. Methods 2001; 48:13–21
  • Trougakos IP, Poulakou M, Stathatos M, Chalikia A, Melidonis A, Gonos ES: Serum levels of the senescence biomarker Clusterin/apolipoprotein J increase significantly in diabetes type II and during development of coronary heart disease or at myocardial infarction. Ex. Gerontology 2002; 37: 1175–1187
  • Jones SE, Jomary C: Molecules in focus Clusterin. The International J. of Bioch. & Cell Biol. 2002; 34:427–431
  • Hidaka S, Kränzlin B, Gretz N, Witzgall R: Urinary Clusterin levels in the rat correlate with the severity of tubular damage and may help to differentiate between glomerular and tubular injuries. Cell Tissue Res. 2002; 310:289–296
  • Chen X, Halberg RB, Ehrhardt WM, Torrealba J and Dove WF: Clusterin as a biomarker in murine and human intestinal neoplasia. PNAS 2003; 100:9530–9535
  • Zhang LY, Ying WT, Mao YS, He HZ, Liu Y, Wang HX, Liu F, Wang K, Zhang DC, Wang Y, Wu M, Qian XH and Zhao XH: Loss of Clusterin both in serum and tissue correlates with the tumorogenesis of esophageal squamous cell carcinoma via proteomics approaches. World J Gastroenterol 2003; 9:650–654
  • Wang L, Erling P, Bengtsson AA, Truedsson L, Sturfelt G, Erlinge D: Transcriptional down-regulation of the platelet ADP receptory P2Y12 and Clusterin in patients with systemic lupus erythematosus. J. of Thromb. And Haemost. 2004; 2:1436–1442
  • Patel NV, Wei M, Wong A, Finch CE, Morgan TE: Progressive changes in regulation of apolipoproteins E and J in glial cultures during postnatal development and aging. Neuroscience Letters 2004; 371:199–204
  • Kim BM, Kim SY, Lee S, Shin YJ, Min BH, Bendayan M, Park IS: Clusterin induces differentiation of pancreatic duct calls into insulin-secreting cells. Diabetologia 2006; 49:311–320
  • Kruger S, Mahnken A, Kausch I, Feller AC: Value of Clusterin immunoreactivity as a predictive factor in muscle-invasive urothelial bladder carcinoma. Urology 2006; 67:105–109
  • Stejskal D, Fiala R: Evaluation of serum and urine Clusterin as a potential tumor marker for urinary bladder cancer. Neoplasma 2006; 53:343–34
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