Type
Sandwich ELISA
Applications
Serum, Urine, Plasma
Sample Requirements
5µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0,16 - 10 ng/ml
Limit of Detection
10 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 3.9%
Inter-assay (Run-to-Run)
n = 8,
CV = 7%
Spiking Recovery
101%
Dilution Linearity
102%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures canine uromodulin
Assay format is 96 wells
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Renal disease
Summary
It has been suggested that uromodulin protein may be an important component of epithelia, which absorb sodium and chloride ions but are impermeable to water. Uromodulin interacts with other molecules and cells including IL-1, IL-2, TNF, IgG, neuthrophils, lymphocytes and monocytes. Uromodulin also acts as a host defense factor against urinary tract infections, specifically targeting type 1 fimbriated Escherichia coli. Uromodulin also acts as an inhibitor of stone formation in healthy individuals by trapping crystals in the same manner. However, this function may be subverted under some circumstances and uromodulin may facilitate crystal aggregation and then promote stone formation. It means that it has been proposed to play a dual role as promoter or inhibitor of nephrolithiasis. Previous reports have described urinary uromodulin as biomarker for distal tubular dysfunction in dogs. Its potential use is illustrated by decreased urinary uromodulin in dogs with chronic kidney diseases (CKD) and in dogs with urolithiasis. The high metabolic activity of the cells of the thick ascending limb of Henle’s loop makes them particularly vulnerable to ischemic injury. Consequently, in acute kidney injury (AKI) caused by ischemic insults, evaluation of uromodulin as an early AKI biomarker is potentially valuable. The study on the Bernese mountain dogs concluded that uromodulin together with the other identified urinary proteins may be valuable markers for the diagnosis of juvenile nephropathy.
Instructions for Use (RUO)
Instructions for Use (RUO)
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