Type
Sandwich ELISA
Applications
Serum, Urine, Plasma
Sample Requirements
20 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
25 - 800 pg/ml
Limit of Detection
4 pg/ml
Intra-assay (Within-Run)
n = 8,
CV = 4.2%
Inter-assay (Run-to-Run)
n = 8,
CV = 7.1%
Spiking Recovery
94.0%
Dilution Linearity
98.0%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures specifically canine procalcitonin
Assay format is 96 wells
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Immune Response, Infection and Inflammation, Sepsis, COVID-19
Summary
Procalcitonin (PCT), a polypeptide with a molecular mass of about 13 kDa, is the precursor of calcitonin. PCT is constitutively produced in the C cells of the thyroid gland and does not exhibit hormone activity.
PCT is markedly elevated within 2 to 4 hours in severe forms of systemic inflammation or in bacterial infections, and the level persists until recovery. The biological half-life of PCT is 22 to 26 hours, an advantageous time point compared with CRP and other acute-phase reactants. Because up-regulation of PCT is attenuated by INF- γ, a cytokine released in response to viral infections, PCT is more specific for bacterial infections and may help to distinguish bacterial infections from viral illnesses.
It should be noted that PCT is also elevated in noninfectious conditions such as trauma, surgery, cardiogenic shock, burns, heat stroke, acute respiratory distress syndrome, infected necrosis after acute pancreatitis, and rejection after transplantation.
Severe bacterial infections can result in marked morbidity and death in veterinary patients, with 50–70% of dogs with sepsis succumbing to their disease. Early diagnosis of infection is essential for the appropriate management of sepsis, as it allows rapid administration of antibiotics resulting in improved outcomes. Although PCT mRNA expression from nonthyroidal tissue has been demonstrated in dogs with inflammation, sepsis and SIRS, very little is known about serum PCT concentration in dogs due to the lack of a validated canine assay.
Instructions for Use (RUO)
Instructions for Use (RUO)
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