Type
Sandwich ELISA
Applications
Serum, Urine
Sample Requirements
5 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.31 - 10 ng/ml
Limit of Detection
5 pg/ml
Intra-assay (Within-Run)
n = 8,
CV = 6.3%
Inter-assay (Run-to-Run)
n = 8,
CV = 5.7%
Spiking Recovery
104%
Dilution Linearity
91%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures specifically canine cystatin C
Assay format is 96 wells
Quality Controls are serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Neural tissue markers, Renal disease
Summary
Cystatin C is the most important endogenous serum marker of renal function assessment. Cystatin C evaluation is able to detect an earlier stage of decreased glomerular filtration rate (GFR) than other parameters (serum creatinine, creatinine clearance etc.) and it is considered particularly useful in patients with a high risk of developing nephropathies. Imbalance between cystatin C and cysteine proteinases is associated with inflammation, cancer, Alzheimer's disease, multiple sclerosis and hereditary cystatin C amyloid angiopathy. An increased level has been found in patients with autoimmune diseases. On the other hand, low concentration of cystatin C presents a risk factor for secondary cardiovascular events.
In veterinary medicine, there are multiple reports of the use of cystatin C in the evaluation of renal function indicating that cystatin C is also the most important serum (urine) marker of renal function assessment in dogs.
Instructions for Use (RUO)
Instructions for Use (RUO)
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