| Cat. No. | Size |
Price |
|
|---|---|---|---|
| Discount IC7300 | 96 wells (1 kit) | $640 |
Sandwich ELISA, HRP-labelled antibody
Stool
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
1.0 ng/ml
n = 10; CV = 5.0 %
n = 10; CV = 7.3 %
94.69 %
106.77 %
Autoimmunity, Diabetology - Other Relevant Products, Inflammatory bowel disease
Human calprotectin is a dimer which consists of the sub units S100A8 (10.835 kDa) and S100A9 (13.242 kDa). The monomers are able to bind calcium. The complex is located in the cytosol of neutrophils and is excreted to the intestine during inflammation. The concentration in faecal samples correlates with the severity of inflammatory processes in the intestine. The complex is resistant against enzymatic degradation. The measurement of Calprotectin represents an easy non-invasive analysis of intestinal inflammation with the possibility to differentiate between noninflammatory irritable bowel disease and intestinal inflammation accompanied with morphological alteration of the intestinal mucosa.
In case of viral or bacterial infections of the gut the concentration of calprotectin in faeces is increased. Therefore, before further diagnostic clarification of a chronic inflammatory bowel disease and in case of an appropriate / corresponding clinic, the exclusion of an infectious cause (e.g. detection of pathogens, detection of pathogen-specific antibodies) is always necessary.
The use of non-steroidal anti-inflammatory drugs (e.g. aspirin, ibuprofen, diclofenac) or COX-2 inhibitors (e.g. celecoxib) can lead to enteropathies that lead to an increase in the calprotectin level in the stool. Before carrying out the determination, the corresponding medication should therefore not be taken for a period of 14 days if possible so as not to influence the measurement of the degree of intestinal inflammation. Administration of pantoprazole (20 mg/day) can lead to increased calprotectin levels. A measurement of calprotectin should be carried out 3 weeks after discontinuation of the medication.
Applications:
The ImmuChrom complete calprotectin kit allows an easy, rapid and precise quantitative determination of calprotectin in biological samples. The kit includes all reagents ready to use for preparation of the samples.