Type
Competitive ELISA, Immobilized antigen
Description
For the direct quantitative determination of β2 Microglobulin by enzyme immunoassay in human serum.
For research use only.
Applications
Serum
Sample Requirements
20 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.2–10 mg/L
Limit of Detection
0.1 mg/L
Intra-assay (Within-Run)
CV = 4.9%
Inter-assay (Run-to-Run)
CV = 6.7%
Features
- RUO
- calibration range 0.2-10 mg/L
- limit of detection 0.1 mg/L
- intra-assay CV = 4.9%
- inter-assay CV = 6.7%
Research topic
Oncology, Renal disease
Summary
β2-microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β2-M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.
Clinical Trends:
− Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M.
− β2-M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
− Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M.
β2-M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2-M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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