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Anti-KLH Rat IgM ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Keyhole limpet hemocyanin
  • Species:Rat
Cat. No. Size Price


633-13769/639-13761 96 wells (1 kit) $715
PubMed Product Details
Technical Data

Cat # changed from 633-13769 to 639-13761

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma

Sample Requirements

50 μl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.13–200 ng/ml

Limit of Detection

3.13 pg/ml

Intra-assay (Within-Run)

CV = 5%

Inter-assay (Run-to-Run)

CV = 5%

Summary

Features

  • Rapid assay (total reaction time:2hours 20min.).
  • A small sample volume.
  • An ecologically excellent preservative is used.
  • Every reagent is provided in liquid form and ready to use.
  • Excellent precision and reproducibility.

Research topic

Immune Response, Infection and Inflammation, Animal studies

Summary

Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, andpolymorpho­nuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

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