| Cat. No. | Size |
Price |
|
|---|---|---|---|
| Discount IC7200 | 96 wells (1 kit) | $675 |
Sandwich ELISA, HRP-labelled antibody
Stool
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
0.01 ng/ml
103.66 %
108.84 %
Immune Response, Infection and Inflammation
The group of ß-defensins are a class of antimicrobial cationic arginine-rich peptides and are part of the innate non-specific immune response. To date, nine different ßdefensins have been identified in humans. Defensins are produced by neutrophilic granulocytes and mucosal cells of the small and large intestine (5). This includes ßdefensin 2, which consists of 64 amino acids and has a molecular weight of 7 kDa. Inflammation and microorganisms result in increased expression of ß-defensins (2). Defensin deficiency is observed in Crohn's disease patients. Due to the restricted barrier function of the intestinal mucosa, the inflammations typical of Crohn's disease can develop through bacterial invasion (3, 4). Defensin deficiency is discussed as a possible cause of the disease (6). Defensins inhibit the activity of histamine-producing germ species (6). In addition, they have a modulating effect on the release of histamine from mast cells in the mucous membrane. At low histamine concentrations they cause release, at high concentrations they have an inhibitory effect (6). Defensin deficiency has an increased susceptibility to bacterial infections of the intestinal mucosa (6) Taking non-steroidal anti-inflammatory drugs (e.g. aspirin, ibuprofen, diclofenac) or COX-2 inhibitors (e.g. celecoxib) can lead to enteropathies, which result in an increase in inflammatory parameters in the stool (8). Before carrying out the determination, the corresponding medication should therefore not be taken for a period of 14 days, so as not to influence the measurement of the degree of intestinal inflammation.
Applications:
The ImmuChrom complete ß-defensin 2 kit allows an easy, rapid and precise quantitative determination of ß-defensin 2 in biological samples. The kit includes all reagents ready to use for preparation of the samples.